Goat Anti-Rabbit IgG (H+L) HRP
Immunohistochemistry Kit
Components of Immunohistochemistry Kit (Goat Anti-Rabbit IgG H&L (HRP)); G1215-200T
G1215-1; DAB diluent; 12.5 mL
G1215-2; 50× DAB stock solution; 250 μL
G1215-3; Goat Anti-Rabbit IgG H&L (HRP);100 μL
Instruction Manual; 1 pc
Product Applications & Validation
Our HRP-labeled Goat Anti-Rabbit IgG (H+L) Kit is ideal for immunohistochemistry (IHC), Western blotting, ELISA, and immunocytochemistry. It provides clear, sharp, and reliable staining for a wide range of rabbit primary antibodies, making it perfect for pathology labs, academic research centers, and biotechnology R&D facilities.
Every batch is rigorously validated across multiple IHC applications to ensure consistent performance and reproducibility in both research and diagnostic workflows. Validation data are available upon request, giving you full confidence in the kit’s reliability.
Operation Steps
Preparation
1. Self-prepared PBS (recommended G4202, G0002), Nucleus counterstaining reagent (recommended hematoxylin stain solution G1004, hematoxylin differentiation solution G1039, Hematoxylin returning blue solution g1040), gradient alcohol, xylene, sealing agent, etc.
2. Preparation of secondary antibody working solution: Goat Anti-Rabbit IgG H&L (HRP) was diluted with PBST (pH 7.2-7.4) in the ratio of 1:200 to obtain the secondary antibody working solution. Ready to use within 48 hours.
3. Prepare DAB color developing working solution: Add 20μL 50×DAB stock solution to every 1 ml DAB diluent , mix well and set aside. Prepared on demand, ready to use.
Operation Steps
1. According to the routine IHC steps, after dewaxing, antigen repair, blocking and primary antibody incubation, the tissue sections were washed with PBS three times for 5 minutes each time.
2. Secondary antibody incubation: add 50-100μL secondary antibody working solution on each slice, completely covered the tissue and incubated at room temperature for 30-60 min. Wash three times with PBS for 5min each time.
3. DAB chromogenic reaction: add 50-100μL DAB working solution on each section, incubated at room temperature for several minutes. The color developing time is controlled under the microscope. To the desired effect, and then immediately wash with water to terminate the color development.
4. (OPTIONAL)Nucleus counterstaining: the sections are counterstained with hematoxylin stain solution for about 3-5 minutes; washed with tap water; differentiated with hematoxylin differentiation solution for 3-5 seconds; washed with tap water; treated with hematoxylin bluing solution; washed with running water for 3-5 seconds.
5. Dehydration and mounting: Sections were dehydrated by gradient alcohol in the usual steps, transparent with xylene, and sealed with neutral gum.
Note
1. If the background is too deep during the IHC chromogenic reaction, consider to extend the washing time, use appropriate blocking solution for mounting, inactive the endogenous catalase, shorten the chromogenic reaction time, reduce the concentration of secondary antibodies, etc. If there is no chromogenic reaction or the chromogenic reaction is too light, the concentration of primary antibodies and secondary antibodies can be properly increased, the chromogenic reaction time can be extended. Second, secondary antibodies were tested for normal color development.
2. DAB is harmful to humans, when operating be careful, and be protected from direct contact or inhalation
For Research Use Only.
Why Choose Our Goat Anti-Rabbit IgG (H+L) HRP Kit?
High Specificity and Sensitivity: Produced from high-quality antigen-purified goat anti-rabbit total IgG, this kit is carefully screened and purified to minimize background signals and maximize staining clarity and contrast.
Wide Compatibility: Works seamlessly with paraffin-embedded tissues, frozen sections, and tissue microarrays, and is compatible with standard antigen retrieval and blocking protocols.
Stable and Consistent Performance: Optimized storage buffers ensure the antibody remains active and reliable even after long-term storage or multiple freeze-thaw cycles.
Strict Quality Control: Each lot is tested with SDS-PAGE, purity verification, and background staining assessments to ensure consistent, high-quality results.
Expert Support and Customization: Our dedicated technical team provides usage guidance, optimization advice, and custom labeling options such as FITC, immunofluorescence, or alternative host species to meet your specific experimental needs.
Immunohistochemistry Kit (Goat Anti-Rabbit IgG H&L (HRP))
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